Biomedical Science Articles

Abstract

The resolution of #alkalineproteins by 2D-polyacrylamide #gelelectrophoresis (2D-PAGE) is often accompanied by protein streak artifacts. To overcome this type of artifact formed during traditional 2D-PAGE, and to further improve the resolution of alkaline proteins, we developed a DeStreak ™ protocol that essentially eliminates the streaking artifact through the use of #hydroethyldisulfide (HED) throughout the entire IEF run. We found that HED improves the separation and resolution of individual basic #proteins when using a narrow range (pH 7-10) IPG strip format.

For more articles on BJSTR Journal please click here:  https://biomedres.us/

For more Biomedical Science Articles on BJSTR

https://biomedres.us/abstracts/BJSTR.MS.ID.001131.php

https://biomedres.us/fulltexts/BJSTR.MS.ID.001131.php

Optimization Strategies for Improving the Resolution of Alkaline Proteins Using 2D-Gel #Electrophoresis by Robert J Hickey in BJSTR