Chemical Analysis of #Bioactive Compound Produced by #Lactobacillusreuteri Isolated From #Domestic Chickens in Iran by Baserisalehi majid in BJSTR
Abstract
The purpose was Chemical analysis of the bioactive compounds produced by Lactobacillus reuteri isolated from domestic chickens. In
addition, the antimicrobial property of the bioactive compounds was
assessed against pathogenic bacteria. A total twenty two samples were
collected from digestive tracts of domestic chickens and subjected to
microbiology analysis. The bioactive producing bacterium was identified
and the bioactive compound was analyzed by SDS-PAGE and HPLC methods.
Finally, antimicrobial property of the bioactive compound against
the pathogenic bacteria was assessed. The results obtained indicated
that the bioactive compound producing bacterium was Lactobacillus
reuteri. The results obtained from SDS-PAGE indicated that the
molecular weight of purified bioactive compound produced by the
isolatewas3.5<
kDa. The bioactive compound showed antimicrobial effect against Escherichia coli, Salmonellatyphimurium, Staphylococcus aureus and Bacillus
cereus. Salmonella typhimuriumi and cereus were more and less sensitive to the bioactive compounds. Overall, chemical analysis of the bioactive
compounds illustrated that the bioactive compound produced by Lactobacillus reuteri was probably Lactocins.
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