Effect
of Local Administration of Voltage-Gated Calcium Channels Blocker
(Gallopamil) Combined with Silicon Conduit on Transected Sciatic Nerve by Mohammad Velayati in BJSTR
Abstract
Background: The outgrowth of regenerating axons of a transected peripheral motor nerve is a slow process. The objective was to assess
the effect of locally administered gallopamil on transected peripheral nerve regeneration and functional recovery.
Methods: Sixty male healthy white Wistar rats were divided
into four experimental groups (n = 15), randomly: In transected group
(TC),
left sciatic nerve was transected and stumps were fixed in the adjacent
muscle. In treatment group defect was bridged using silicone tube (SIL/
Gallopamil) filled with 10 μL gallopamil (100 ng/mL). In silicone
conduit group (SIL), the tube was filled with phosphate-buffered saline
alone.
In sham-operated group (SHAM), sciatic nerve was exposed and
manipulated. Each group was subdivided into three subgroups of five
animals
each and regenerated nerve fibers were studied 4, 8 and 12 weeks after
surgery.
Results: Behavioral testing, biomechanical studies, sciatic
nerve functional study, gastrocnemius muscle mass and morphometric
indices
confirmed faster recovery of regenerated axons in SIL/Gallopamil than
SIL group (p < 0.05). In immunohistochemistry, location of reactions
to
S-100 in SIL/Gallopamil was clearly more positive than that in SIL
group.
Conclusion: When loaded in a silicone tube gallopamil accelerated and improved functional recovery and morphometric indices of sciatic
nerve. This may have clinical implications for the surgical management of patients after nerve transaction.
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