Heat Stress Induced Alterations in Prostaglandins, Ionic and Metabolic Contents of Sheep Endometrial Epithelial Cells In Vitro by Sukanta Mondal in BJSTR
Abstract
Background: Heat stress is one of the major factors
responsible for reduced fertility in livestock including sheep. The
present study was
aimed to elucidate the effect of heat stress on prostaglandins, ionic
and metabolic contents of sheep endometrial epithelial cell in vitro.
Sheep
whole genitalia containing ovaries were collected immediately after
slaughter and transported to the laboratory. Following washing the
healthy
and non pregnant uterine lumen with HBSS with gentamycin and 0.1% BSA,
HBSS containing 0.3% trypsin was then infused and epithelial cells
were isolated by incubation at 370C for 60min. After cell counting and
viability determination, the epithelial cells were cultured in at 38.5oC
for
24hr (control). Heat stressed cultures were acclimated at 38.50C for 6hr
and then placed at 40.50C for 18 hr.
Results: In vitro heat shock significantly (P<0.05)
increased protein, phosphorous, urea and PGF2α contents in culture
medium whereas
significantly (P<0.05) decreased SOD content as compared to control.
Elevated temperature did not significantly (P<0.05) increase calcium
contents but non-significantly (P>0.05) increase glucose, chloride
and PGE2 levels in culture medium. It was concluded that heat stress
altered
prostaglandins, ionic and metabolic contents of endometrial epithelial
cells in vitro.
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