A Technique to Monitor the Real-Time #Proton Leak Status of Cells by Ruihua Wang in BJSTR
Abstract
Proton leak was a detour of protons from their original path
leading to the reduction of mitochondrial membrane potential. It
leads to the decrease of ATP production per glucose molecule. It
is a form of energy loss [1]. Proton leak was found to reduce the
reactive oxygen species (ROS) level [2]; therefore, it plays a role
in aging [3], diet [4-5] and health [6-9]. A method to monitor the
real-time proton leak in cells would help for the understanding of
physiological system and disease conditions. When protons were
leaked out from mitochondria, it increased the leaking of electrons
from electron transport chain [10]. The leaked electron would
generate electric signal in Microbial Fuel Cells (MFC). Measuring
the electric signal sent out during the process of proton leak would
be a way to quantify thereal-time status of proton leak in live cells.
Theoretically, higher the electric signal means higher is the proton
leak. However, the accuracy of MFC measurements is influenced by
the internal resistance of the apparatus. The internal resistance is
contributed by the activation loss from electron transfer from cells
to the anode, the characteristics of cellular metabolism, the flux of
reactants and ohmic loss [11].
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