Manual preparation of drug concentration gradient is #labor-intensive and
limits the efficiency of a drug screening to some extent, especially
at large scale. In this study, we reported an automatic method to
generate drug concentration gradient in 24-well plate. We loaded drug
solution
in a modified hot #ink-jet printer, designed printing pattern in the WORD
software, customized the concentration gradient in the WORD software
by #RGB coding, and finally printed the drug concentration gradient in
the 24-well plate. A drug concentration gradient can be automatically
generated within 5s in arbitrary pattern. On the basis of this flexible
technology, we developed an ink-jet printer-based drug screening assay.
Concentration gradient of #5-fluorouracil, as well as Mcf-7 cells, was
printed in the 24-well plate, and the inhibition rate of #Mcf-7 cells was
measured by MTT method. This was the first time that hot inkjet printing
technology was used in drug screening.
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