Abstract
The strains of wild #Inonotus #obliquus were used as the materials. An improved #CTAB method of Inonotus obliquus
DNA extraction was
applied to obtaining genomic DNA. And it was used for template then to
optimize #RAPD amplification conditions. The results indicated that:
a volume of 25 μL was used which contained 40ng/μl template DNA, 10pmol
primer, 2mmol/L Mg2+, 1.0 unit domestic Taq DNA #polymerase,
100μmol/L dNTP, others complement with ddH2O. The amplification program was that 1 cycle of 5 minutes at 94oC to initial #denature; 45
cycles of 1 minute at 94oC to denature, 1 minute at 40oC for annealing, 1.5 minutes at 72oC for extension; 5 minutes at 72oC for final extension.
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