Abstract
Objectives: The objective of this study was to characterize the degree of #polyacrylamidehydroxyapatite (PAHA) disc demineralization #invitro based on both optimized areal surface profiling and topography analysis using confocal laser scanning microscopy (CLSM).
Methods: A total of 11 test samples were prepared using modified Association of Official Analytical Chemists (AOAC) methods. Test sample
solution was incubated at 37oC for 150 minutes after inoculation with 1% #Streptococcusmutans
and radioisotope-labeled polyacrylamide
hydroxyapatite (PAHA) discs. The degree of demineralization was measured
based on ³²P released from each #radioisotope-labeled PAHA disc
using CLSM. Image analysis systems (2D and 3D) were used to determine
surface topography and roughness.
Results: Sz (maximum height of the surface) showed a
significant difference compared to conventional mean height parameters,
such as Sa
(mean height of the surface) and Sq (root square mean height). Regarding
the skewness and kurtosis, distinct #demineralization was observed
for sponge cake (p < 0.05). While these food samples showed minimal
differences in released radioisotope ³²P, a relatively distinct
appearance
was observed in CLSM.
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