Abstract
Aim: To investigate the effect of Nd:YAG (1064-nm) laser
irradiation on human #periodontal ligament cells, concerning
#proliferation,
differentiation and certain growth factors secretion: vascular
#endothelial growth factor (VEGF), epidermal growth factor (EGF) and
basic #fibroblast growth factor (bFGF).
Materials and Methods: Primary PDL cell cultures were irradiated with Nd: YAG laser (1064-nm), for 20, 40, 60 and 120 seconds (energy
densities: 2.6-15.8J/cm2) and evaluated after 24 and 72 hours. Controls were not irradiated. The experiment was performed in duplicate. Data
analyzed by Students t-test (p<0.05).
Results: Cell cultures irradiated for 40, 60 and 120 seconds
revealed higher proliferation when compared to controls, 24 and 72 hours
after irradiation. Statistical significance was reached for the group of
60s at 24h and for groups of 40s and 120s at 72h (p<0.05). #Alkalinephosphatase (ALP) levels were also found increased for most of the
irradiated cell cultures compared to controls. Statistical significance
was
reached for the 120s cultures at 24h as well as for the 20s at 72h
(p<0.05). Growth factors’ levels for irradiated cultures and controls
were
arbitrary during the experiment.
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