Abstract
Objective: To establish whether 45-bp insertion (I)/deletion (D) polymorphisms in Uncoupling protein 2 (UCP2) are associated with
moderate and/or severe obesity in a Saudi Arabian population.
Methods: The study enrolled 151 (male and female) subjects
originating from the eastern province of Saudi Arabia, and assigned each
to either a ‘non-obese’, ‘moderately obese,’ or ‘severely obese’ group.
Genomic #DNA was extracted from all subjects, and screened for UCP2
insertion/deletion #polymorphisms using a standard PCR protocol.
Results: The overall frequencies of the UCP2 45-bp
insertion/deletion #genotypes D/D, I/D, and I/I within the analyzed
population were
58.3%, 36.4%, and 5.3% respectively. The D/D genotype was highly
prevalent within the severely obese group (82.9 %) compared to the
non-obese (46.2%) and moderately obese (53.3%) groups. Using a dominance
model, the conducted logistic regression analysis showed no
significant association between the insertion allele and moderate
obesity (OR = 0.75, 95% CI: 0.35–1.59, P = 0.585); however, in contrast,
a
strong association was found between the deletion allele and severe
obesity (OR= 0.18, 95% CI: 0.07–0.44, P = 0.0004).
Conclusion: The present study reported the frequency of the
UCP2 45-bp insertion/deletion polymorphisms in a population originating
from eastern Saudi Arabia, and furthermore, identified a strong
association between the D/D genotype and severe #obesity in this
population.
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