Abstract
#Monolayercultures of #epithelial or gingivalfibroblasts have been
used for the study of #oralmucosa and the effect of external stimuli,
such as different types of dental materials. However, monolayer
models lack polarized cell phenotype and systemic components,
which affect their function and response to #stimuli [1,2]. Several
tissue-engineered oral mucosa models have been developed to
overcome these limitations with applications in different fields [2-
4]. These 3D cultures provide a higher degree of complexity than
monolayer cell cultures being closer to explant cultures, providing
an #invitro model resembling the in vivo situation [2]. At least two
tissue-engineered oral mucosa models are commercially available,
SkinEthicTMOral Epithelium and Gingival Epithelium constructs
from EPISKIN (Lyon France), or the Epi OralTM and Epi GingivalTM
constructs from Mat Tek Corporation (Ashland, MA, USA). Other
commercial skin tissue models are validated to be used as an
alternative to animal testing to proof cosmetics safety [5]. Thus it
could be envisaged that in the near future oral tissue models might
be approved to test cosmetic dental raw materials or formulations
such as tooth pastes or mouthwashes. However, the existing
commercial models lack a #fibroblast/#collagenmatrix component,
which is critical in human oral mucosa [2].
#Tissue-Engineered Oral #Mucosa Constructs for in Vitro Research and Clinical Applications by Joana Maria Ramis Morey in BJSTR
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