Physical Medicine and Rehabilation

Abstract

#Morphology of #filamentousfungi in submerged #fermentation has a huge impact on product yield. In order to discover the genes related with pellets formation, the method of insertion mutation by T-DNA was employed to construct the #mutagenesis library of marine #Aspergillusniger. In this study, 125 positive transformants were identified by detecting the expression of enhanced green fluorescence and one mutant easier to pelletize was selected by flask-shaking test. The insertion site of T-DNA, determined by hiTAIL-PCR, was located in the promoter of plasma membrane ATPase gene. The mutant exhibited decreased production of spores, weaker salt- and acid-tolerance and a broader range of optimal growth temperature

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Enhancement of Pellets Formation of Marine Aspergillus Niger with Deficiency of Plasma Membrane ATPase by Shan Jing Yao in BJSTR