Abstract
#Morphology of #filamentousfungi in submerged #fermentation has a huge
impact on product yield. In order to discover the genes related
with pellets formation, the method of insertion mutation by T-DNA was
employed to construct the #mutagenesis library of marine #Aspergillusniger. In this study, 125 positive transformants were identified by
detecting the expression of enhanced green fluorescence and one mutant
easier to pelletize was selected by flask-shaking test. The insertion
site of T-DNA, determined by hiTAIL-PCR, was located in the promoter of
plasma membrane ATPase gene. The mutant exhibited decreased production
of spores, weaker salt- and acid-tolerance and a broader range of
optimal growth temperature
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