Journals on Nuereological Disorders

Abstract

The growth of #gram-negativeanaerobes requires the complete absence of oxygen (0.001%), and is usually treated in an anaerobic chamber.However, this traditional method is costly and time consuming.In this study, we describe a new, rapid method for the culture of #anaerobicbacteria, such as #P. gingivalis, and #P. endodontalis, in a rotary shaker under anaerobic conditions, by sealing the bacteria growth media from oxygen exposure using a mineral oil overlay. By using these cells, we found P. gingivalis or P. endodontalis mediated different signaling pathways in RAW264.7 cells.

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A New Method for the Cell Culture of Anaerobic Bacteria: P. gingivalis- or P. endodontalis-Mediated Pathways in RAW264.7 Cells by Xiaoen Tang in BJSTR