In this paper, we present a study of stimulated Brillouin scattering
(SBS) by #E-coli, B.subtilis and #coliphages in water. We found the
Lamb-like dip in the spectra of SBS in the presence of organisms in
water (higher than 1 cells/ml) at excitation by laser radiation with #wavelengths 810 and 1017 nm and power 40¬150 mW. The mathematical model
of non-linear interaction of the laser radiation with double-stranded
DNA, consisted in two #antiparallel chains, was designed to explain this
phenomenon. We developed a new method based on #coherent spectroscopy and created an
instrument for controlling #pathogenic microorganisms in the flow of
water [1]. However, that our method allowed only determining the
presence of a given microorganism [2]. At the same time, it is of
interest to develop an instrument that not only estimates the
concentration of the pathogen, but can also be integrated into an
automatic line monitoring water condition. It is known that the
automatic determination of the concentration of a given microorganism
presented a challenge due to complexity of the processes of interaction
of the coherent radiation with the #DNA structure. Therefore, in order to
create such an instrument and automate the process of monitoring and
also determine the concentration of the pathogen, it is necessary to
create efficient algorithms for processing incoming information.
Building a reliable algorithm for recognizing the patterns of spectral
distributions requires to carry out not only a statistical processing of
spectra, but also a modeling of physical processes of interaction of
DNA of the pathogenic organism with the propagating coherent radiation.
However, the sufficient theoretical justification for this method has
not yet been developed.
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