Journals on Medical Drug and Therapeutics - BJSTR Journal

Abstract

This study aimed to analyze the effects of #semen cryopreservation on #seminal proteins. Initially, we analyzed the effects of cold shock and the advent of proteomics and its benefits on the characterization of seminal proteins. Subsequently, studies reporting losses of some sperm proteins and increase in the expression of others during the cooling and freezing of the semen of different species are presented. Finally, some questions were raised to motivate the study of semen proteomics aiming to predict quality losses during the cryopreservation of semen in breeding animals of greater genetic merit. There have been reports of resistance of sperm to the freezing process since 1776 with Spallanzani. However, it was only with the discovery of the #cryoprotectant effects of #glycerol by Polge et al. [1] that sperm cryopreservation began to be disseminated. The process consists of dilution, cooling, freezing, storage, and defrosting. Moreover, the interaction between the diluent, cooling and freezing curves and thawing is required to obtain semen with good fertilizing capacity Amann et al. [2]. Cold shock occurs when semen is rapidly cooled from room temperature to 0°C. It is characterized by circular movement of spermatozoa, premature loss of motility, decreased energy production, and increased membrane permeability with consequent loss of intracellular ions and molecules. Damage to the sperm membrane is responsible for these changes Amann et al. [2]. One way to reduce these effects is to slowly cool the diluted semen and add milk or egg yolk proteins to semen #diluents Amann et al. [2]. With the advent of #proteomics, many studies aiming to characterize seminal proteins were conducted, which allowed identification of changes in protein composition after cryopreservation and correlation of these changes with the fertility and freezing patterns of the semen of each animal.

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Seminal #Proteins and Cryopreservation of Semen: A Mini-Review by Deiler S Costa in BJSTR