Friday, August 16, 2019

Immunological Diseases - BJSTR Journal

Abstract

Banana is an important crop and fruit in many developing countries. China is one of the main banana-producing countries. China's banana production was always heavily affected by typhoon. The physical strength of #banana pseudostem was mainly determined by the content of lignin. 4-coumarate: CoA ligase (4CL) is a rate-limiting enzyme for lignin synthesis. There were 25 4CL genes in banana A genome. Mu4CL15 was the main gene for encoding 4CL in banana pseudostem. The substrate that Mu4CL15 preferred can be ordered as the followed: 4-coumaric acid, caffeic acid, #ferulic acid and #sinapic acid from the most to the least. After Mu4CL15 was transformed into tobacco, the lignin content in the stem of the transgenic plant was significantly higher than that of the wild type. The stem strength of the transformed seedlings was higher than that of the wild type. After banana embryogenic suspension cells were transformed with Mu4CL15, the lignin content in pseudostem of the transformed plant was significantly higher than the control. The physical strength of the transformed-plant pseudostem was also distinctly higher than the control. Enhancing banana plant's lodging resistance by transforming 4-coumarate: #CoA ligase gene is feasible. Besides transgenic method, screening and identifying the #germ plasm resource of banana can also breed new cultivars with higher resistance to lodging.

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