Journals on Medical Microbiology - BJSTR Journal

Abstract

Cell culture is #indispensable for in vitro studies. Since in vivo experiments are carried out with organisms consisting of a whole #cell, any invasive procedure is pre-determined, and the allowance of the ethical committee is applied, which delays your study. Sometimes quick experiments are needed to see the effect of a specific substance. In this case, cell culture assays allow us to quickly visualize the impact of a substance in vitro giving us the opportunity to utilize the target cell type in the body. However, there are some common mistakes made in cell culture. As is known, when a substance is tested in cell culture, the #stock solution is first prepared. This solution is usually DMSO because of its ability to dissolve a wide range of substances. And the stock solution prepared should be at least 100 times the size of the dose to be given to the cells. This is a dilution method for reducing the #toxic dose of DMSO. In spite of that of that non-toxic dose of DMSO is 0.1%, 100-fold diluted #DMSO is acceptable as long as untreated control with DMSO is also used.However, when the stock solution is being prepared, the final volume is not considered. For example, assume that you will prepare 1 ml and 100 mM of stock solution from substance X.

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Small but Critical Mistakes Commonly Made in Cell Culture Experiments: An Opinion Based on Observation and Experience by Sedat Ka�ar in BJSTR