Abstract
#Lipases are produced extracellular by the bacterial strain #Bacillus subtilis in submerged state fermentation was purified from the cell free
culture broth by ammonium sulphate precipitation at 70% saturation
followed by purification solvent precipitation by acetone and ethanol.
DS- PAGE analysis indicated that the enzymes #protein molecular mass was
19.4 kDa. The enzyme activity was maximum growth 72 hours of incubation
time, with an activity (0.95U/ml). Lipase activity was maximal at 1%
concentration of the substrate. Olive oil produced (32.67) units of
lipase was the best induces for lipase production enzyme activity. The
optimum PH 8 for the activity of lipase. Lipase is produced by various #microbes, bacteria, fungi, yeast, mammals
and plants in large amounts. Lipase have been used extensively is oleo
chemical industry and dairy industry [1] and to produce the structural
triglycerides [2]. #Extracellular lipase produced by microorganisms is
being investigated for its potential application in various industrial
processes like detergents, oils, fatty acids and diary coupled with
#enormous therapeutic uses [3].
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Extra Cellular Lipase Production, Purification and Characterization by Using Bacillus Subtilis in Submerged State Fermentation by Leena Grace Beslin in BJSTR
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