Microarray Analysis of Differentially Expressed LncRNAs with Associated Co-Expression and CeRNA Networks in #CoronaryHeartDisease by Siying Wu in BJSTR

Abstract

Objectives: The present study aims to explore the expression profiles and biological functions of long-chain #noncodingRNA (lncRNA) in coronary heart disease (CHD) and to construct a lncRNA/microRNA (miRNA)/messenger RNA (mRNA) network for mechanism exploration.

Methods: Ten human blood samples (five each from the CHD and control groups) were included. The differentially expressed lncRNAs, miRNAs, and mRNAs were evaluated using #microarray. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for differentially expressed lncRNAs–mRNAs was performed. The miRNAs that interact with differentially expressed #lncRNAs were predicted using the miRanda package, whereas the mRNAs with clear biological functions and regulated by miRNAs were predicted using miRWalk2.0. Expression networks, including coding/noncoding gene #co-expression, cis-regulation, and lncRNAs-#transcription factors (TFs), were constructed using #bioinformatics methods and were then combined to form a lncRNA-miRNA-mRNA network.

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Microarray Analysis of Differentially Expressed LncRNAs with Associated Co-Expression and CeRNA Networks in #CoronaryHeartDisease by Siying Wu in BJSTR