Simple, Rapid and Effective Separation of Nuclear #RedBloodCells from Peripheral Blood of Pregnant Women: Preliminary Study by Masato Kantake in BJSTR

Abstract

The analysis of #nuclearredbloodcells (NRBCs) in pregnant woman is believed to be a practical, noninvasive prenatal test that is much useful for understanding the prenatal fetal status. Here, we show a simple, rapid and effective method to separate NRBC from maternal peripheral blood with preterm labor. We used MACS Technology which is based on MACS #MicroBeads, highly specific antibodies coupled to super paramagnetic 50nm particles, MACS Columns containing a matrix of #ferromagneticspheres, and MACS Separators providing a strong magnetic field. This method could enrich the CD45-CD71+ cells from 0.3% of whole blood cells to 93% of separated cells. When the fetal cell ratio in these separated cells is high in some extent, our method may be useful for further analysis. The yield of NRBCs from 2ml of maternal blood by this method is enough to be used for not only targeted DNA #methylavtion analysis but whole exome and whole #transcriptomeanalysis using next generation sequencer which may lead to progress in #perinatology.

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Simple, Rapid and Effective Separation of Nuclear #RedBloodCells from Peripheral Blood of Pregnant Women: Preliminary Study by Masato Kantake in BJSTR