Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an #Amperometric H2O2 Biosensor by AKM Kafi in BJSTR

Abstract

A novel #amperometric H₂O₂ biosensor based on #immobilization of catalase with polymerized MPNFs of SnO2 onto glassy carbon electrode with chitosan have been proposed in this work. #Multiporousnanofibers of SnO2 were synthesized by #electrospinning method from the tin precursor by controlling the concentration followed by polymerized with aniline. Catalase was then co-immobilized with the polymerized nanofibers on the surface of glassy carbon electrode by using chitosan. The polymerized MPNFs of SnO₂ play a significant role in facilitating the electron exchange between the #electroactivecenter of catalase and the electrode surface. Cyclic Voltammetry and #amperometry were used to study and optimize the performance of the fabricated H₂O₂ biosensor. The PANI/SnO₂-NFs/Catalase/Ch/GCE biosensor displayed a linear amperometric response towards the H₂O₂ concentration range from 10 to 120 μM with a detection limit of 0.6 μM (based on S/N=3). Furthermore, the biosensor reported in this work exhibited acceptable stability, #reproducibility, and #repeatability.

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Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an #Amperometric H2O2 Biosensor by AKM Kafi in BJSTR