Saturday, February 23, 2019

Biomedical Science Articles

Abstract

The resolution of #alkalineproteins by 2D-polyacrylamide #gelelectrophoresis (2D-PAGE) is often accompanied by protein streak artifacts. To overcome this type of artifact formed during traditional 2D-PAGE, and to further improve the resolution of alkaline proteins, we developed a DeStreak â„¢ protocol that essentially eliminates the streaking artifact through the use of #hydroethyldisulfide (HED) throughout the entire IEF run. We found that HED improves the separation and resolution of individual basic #proteins when using a narrow range (pH 7-10) IPG strip format.

For more articles on BJSTR Journal please click here:  https://biomedres.us/



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