Introduction: #Monocytes infiltrate tissues and differentiate
into #tissue-specific macrophages by interaction with other cells in
tissues. Macrophages in the arterial wall uptake of oxidized LDL and
form foam cells and induce inflammatory changes in tissues by secreting
inflammatory cytokines. Chronic inflammation is believed to be involved
in the development of cancer and lifestyle-related diseases. Whereas, in
#human monocytes, the mRNA expression of inflammatory factors increases
by interactions with cancer cells however, this increase can be
suppressed by pretreatment with low-dose LPS. In the present study, we
investigated changes in the gene expression of some key cytokines,
inflammatory factors [IL-1β and #adiponectin] and a chemotactic factor
[MCP-1], after interactions between human adipocytes and LPS-pretreated
human monocytes.
Materials and Methods: The human monocyte cell line THP-1 was
treated with LPS and subsequently co-cultured with human adipocytes
using an insert co-culture system. The gene expressions of inflammatory
factors and chemotactic factor were analyzed using quantitative
real-time PCR and DNA microarray.
Results: The increased mRNA expression of IL-1β in
human adipocytes after co-culture was suppressed by interaction with
LPS-pretreated THP-1 cells. The decreased mRNA expression of #adiponectin
in human adipocytes after co-culture was increased by interaction with
LPS-pretreated THP-1 cells. In addition, the increased mRNA expression
of MCP-1 in THP-1 cells after interaction with human adipocytes was
suppressed by LPS-pretreatment.
Conclusion: #LPS-pretreated human monocytes may have
anti-inflammatory effect in adipose tissues. LPS-treated human monocytes
may be beneficial for the prevention of diseases caused by chronic
inflammation.
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