Journals on Biomedical Engineering - BJSTR Journal

Abstract

PCR or #Polymerase Chain Reaction is a technique widely used in molecular biology laboratories. Since it was developed in 1983, many procedures use this technique as a basic principle, for example: forensic tests, diagnosis of diseases, #phylogenetic studies, identification of mutations, nucleotide sequencing, among others [1-3]. In our procedures of IGF-I diagnostic, we use the techniques of northern blot, western blot, Elisa, #immunocytochemistry and cytofluorometry, and the technique of PCR seems still the most rapid and useful in clinical laboratory [4,5] The IGF-I test is among the most important indications for precancerous diagnostic [6]. Therefore, the accessibility of PCR technique giving increased efficiency and rapidity has become a goal to establish a new methodology. The traditional thermal cycler consists of a metal plate or block that has the #Eppendorf tubes located at the top, and at the bottom is an electrical device that uses #Peltier technology to increase or decrease the temperature according to the requirements of the cycle. There are temperature sensors distributed below the block (Figure 1).

 For more articles on BJSTR Journal please click on https://biomedres.us/

For more Biomedical Engineering Articles on BJSTR

https://biomedres.us/fulltexts/BJSTR.MS.ID.001678.php 

New Approach of PCR Technology for IGF-I Evaluation by  Jerzy Trojan in BJSTR