Journals on Biomedical Imaging - BJSTR Journal

Abstract

Introduction: #Natural killer (NK) cells are central components of the #innate immunity. They have ability to kill a wide range of cancer cells and are a promising tool for both autologous and allogeneic immune enhancement therapy in cancer treatment. Actually, NK cells can be derived from multiple sources such as: peripheral blood, cord blood... Among these, cord blood (CB) is known as an ideal for NK cells expansion because it occupies a higher percentage of NK cells compare to the peripheral blood and is a rich source of #hematopoietic stem cell as well as progenitor immune cells. The objectives of this study were to invent a reasonable approach for expanding a relevant number of NK cells from human cord blood to clinical application. Methods: At the initial step, cord blood was collected from the patients and then the mononuclear cells were obtained by #density centrifugation with Ficoll. After that, these cells were cultured in 2 stages: stimulation and expansion with inactivated #autologous plasma and BINKIT expansion kit contains several kinds of growth factors which are specified for NK cells. The immunophenotype of NK cells was analyzed every 2 days through %CD56+CD3- by Flow cytometry technique. The ex-vivo activation and expansion of NK cells was performed in GMP grade -clean room for about 3 weeks.

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Efficient Expansion of Human Umbilical Cord Blood- Derived NK Cells Ex Vivo without Requiring Feeder Layers by Hoang Thi My Nhung in BJSTR