Journals on Biomedical Imaging - BJSTR Journal

Abstract

Identification of cost-effective methods of cell lysis and lipid extraction from microalgae represent a critical step in the determination of promising biodiesel-producing species. Several cell disruption methods were tested in the microalgae #Amphora subtropica, Tetraselmis marina #Picochlorum sp,. and Dunaliella sp. Among the methods tested, enhanced lipid extraction was achieved through microwave for Amphora biomass and osmotic shock for Tetraselmis, #Picochlorum, and Dunaliella biomass. The #Cequier-Sanchez method rendered the highest TL content in Amphora subtropica and #Picochlorum sp. while Bligh and dyer method gave the highest TL content in Tetraselmis marina and Dunaliella sp. The treatment of microalgae with 5 days of UV-B stress led to a carotenoid and lipid content increase of 44, 159, 116 and 150%, respectively, and of 56, 84, 111 and 80%, respectively, but with a reduction of the total chlorophyll content (30, 43, 24 and 34%, respectively). Furthermore, a significant increase in #malondialdehyde level and #superoxide dismutase, catalase and #glutathione peroxidase activities toward the control was recorded in response to UV stress. 

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Production of Lipids from Tunisian Wild Microalgae: Influence of Cell Disruption, Extraction Methods and UV-B Radiation Exposure by Ines Dahmen Ben Moussa in BJSTR