Journals on Medical Microbiology - BJSTR Journal

Abstract

Rapid identification of bacterial species causing an infection has shown to have positive impact on the clinical outcome. #MALDI-TOF MS is a viable and rapid method for identification of bacterial species not only on agar plates but also directly from positive blood culture bottles and can thus shorten the turnaround time significantly. The aim of this study was to evaluate MALDI-TOF MS on liquid enrichment medium as a possible method for rapid identification of #bacterial species by comparing it to routine culturing procedures. The broth was harvested when growth was suspected and analyzed using MALDI-TOF MS. An overall sensitivity of 70.4% was achieved when comparing MALDI-TOF MS and routine procedures. In conclusion, the direct identification from the broth showed a substantial degree of correlation to the routine procedures, where identification was performed from the colonies growing on the agar plates. To correctly diagnose and treat bacterial infections the type of bacteria causing the #infection needs to be accurately identified [1,2]. Rapidly identifying the bacterial species causing an infection has shown to positively impact both the clinical outcome as well as the #healthcare costs [3]. MALDI-TOF MS has in recent years become a relatively cheap and easy method of identifying bacteria from solid media and is widely used in clinical laboratories [4,5].

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Rapid Identification of Bacterial Species Directly from Enrichment Broth By MALDI-TOF Mass Spectrometry  by Baharak Saeedi in BJSTR

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