#Helicobacter pylori (H. pylori) is a colonizer in the gastric mucosa
of more than 50% of the human population.
Complications due to this bacterium include chronic gastritis, gastric
#mucosa-associated lymphoid tissue lymphoma, peptic ulcer and gastric
adenocarcinoma. The aim of this study was molecular detection of H.
pylori in biopsy samples by amplification of conserved sequences of dupA
gene.The in silico study for detection of
conserved region in dupA was performed. Seventy-nine biopsy samples were
collected and DNA was extracted from them. The #polymerase chain reaction
(PCR) was performed to amplify dupA conserved region. The glmM
gene was also amplified as a standard control alongside the dupA gene.Results of 79 samples, 31 and 48 were collected from
males and female patients, respectively. The age range of patients was
13-85
years; Mean±SD (49.68±18.82).The patients’ complications included 35.4%
#reflux (13.92% males and 21.52% females), 32.9% gastritis (7.59
males and 25.31% females) and 8.9% peptic ulcer (2.58 males and 6.32%
females). The PCR amplification of dupA showed 98% and 100%
sensitivity and specificity, respectively, whereas results for glmM were
26% and 100%, respectively.
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