Abstract
#Ovine Amniotic epithelial cells (oAEC) are a subject of placental
stem cells with great regenerative and #immunomodulatory properties.
Indeed, oAEC are object of intense study for regenerative medicine
thanks to the several advantages in developing pre-clinical studies on a
high value translational animal model, such as sheep. For this reason, a
critical standardization of in vitro culture practices is fundamental
in order to maintain during amplification the oAEC native phenotype,
improving both oAEC in vivo therapeutic potential and clinical
outcomes. Here, is described an oAEC culture protocol with
supplementation of Progesterone and Estradiol hormones, able to modulate
the native epithelial phenotype during the in vitro amplification. In
addition, it is described the culture protocol that is able to
differentiate oAECs towards #osteogenic and #chondrogenic lineage.
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For more Medical Microbiology Articles on BJSTR
Protocol for the Ovine Amniotic Epithelial Cell In Vitro Culture and Differentiation by Hashimita Sanyal in BJSTR
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