Introduction: #Fructose is formed in the seminal vesicle under
the action of testosterone and is secreted along with the vas deferens
during each ejaculation, so fructose is considered a #biochemistry that
faithfully reflects the function of these components. Fructose
concentrations in normal semen confirm the role of testosterone and the
function of normal seminal vesicles, without the phenomenon of
obstruction.
Materials and Methods: The semen samples were obtained from
300 male partners of infertile couples who attended the Department
Biomedical and Genetics of the Hanoi medical university and the semen
samples were analyzed for the routine seminal parameters. Fructose
concentration was assessed using #spectrophotometry (improved ROE
method).
Results: Optimal solution concentration of TCA is 10%.
Composite color composition include 2,5 ml of HCL 30% and 0,25 ml of
#resorcinol 0,1%; Repeatability: CV% = 1, 407 % <5%; Intermediate
precision: CV% = 2.032 % < 5%; Trueness: tex= 0,906 <tt = 2.262.
Specificity was 98,9 % and 99,52 % sensitivity. Significant correlation
between the two methods with r=0,990, p<0,001; average difference
between the two methods is 0,0116 with equivalent is 2,078 %
(p=0,035< 0,05).
Conclusion: Complete the manufacturing procedure of kit for determination of fructose concentration in seminal fluid.
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